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edu click-it reaction mixture  (Thermo Fisher)


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    Structured Review

    Thermo Fisher edu click-it reaction mixture
    Edu Click It Reaction Mixture, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/edu+click-it+reaction+mixture/edu+click+it+reaction+mixture/pmc11393457-303-10-21
    Average 90 stars, based on 1 article reviews
    edu click-it reaction mixture - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Maf expression in B cells restricts reactive plasmablast and germinal center B cell expansion.
    Article Snippet: After washing with 2mL BD Perm/Wash (BD Biosciences, Cat. 554723), cells were incubated in 150 μL permeabilization buffer plus (BD Biosciences, Cat. 561651) on ice for 10min followed by wash, and 5min fixation with 150 μL Cytofix/Cytoperm followed by another wash. For DNA digestion, cells were incubated at 37 °C for 1 h in a solution of DNase I at 300 μg/mL in PBS. .. After washing with Perm/Wash, cells were resuspended in EdU Click-iT reaction mixture (Click-iT Plus EdU Alexa 350 Flow Cytometry Assay Kit, Thermo Fisher) and incubated for 20min at room temperature. .. After washing, cells were resuspended in Perm/Wash buffer containing antiCD16/32 antibody, PE-conjugated NP (Bioresearch), anti-CD95-PE-Cy7 (clone, SA367H8, Biolegend), and anti-BrdU-APC (APC BrdU Flow Kit, BD Biosciences, Cat. 557892) and incubated for 30min at room temperature.

    Article Title: Maf expression in B cells restricts reactive plasmablast and germinal center B cell expansion
    Article Snippet: After washing with 2 mL BD Perm/Wash (BD Biosciences, Cat. 554723), cells were incubated in 150 μL permeabilization buffer plus (BD Biosciences, Cat. 561651) on ice for 10 min followed by wash, and 5 min fixation with 150 μL Cytofix/Cytoperm followed by another wash. For DNA digestion, cells were incubated at 37 °C for 1 h in a solution of DNase I at 300 μg/mL in PBS. .. After washing with Perm/Wash, cells were resuspended in EdU Click-iT reaction mixture (Click-iT Plus EdU Alexa 350 Flow Cytometry Assay Kit, Thermo Fisher) and incubated for 20 min at room temperature. .. After washing, cells were resuspended in Perm/Wash buffer containing anti-CD16/32 antibody, PE-conjugated NP (Bioresearch), anti-CD95-PE-Cy7 (clone, SA367H8, Biolegend), and anti-BrdU-APC (APC BrdU Flow Kit, BD Biosciences, Cat. 557892) and incubated for 30 min at room temperature.

    Incubation:

    Article Title: Maf expression in B cells restricts reactive plasmablast and germinal center B cell expansion.
    Article Snippet: After washing with 2mL BD Perm/Wash (BD Biosciences, Cat. 554723), cells were incubated in 150 μL permeabilization buffer plus (BD Biosciences, Cat. 561651) on ice for 10min followed by wash, and 5min fixation with 150 μL Cytofix/Cytoperm followed by another wash. For DNA digestion, cells were incubated at 37 °C for 1 h in a solution of DNase I at 300 μg/mL in PBS. .. After washing with Perm/Wash, cells were resuspended in EdU Click-iT reaction mixture (Click-iT Plus EdU Alexa 350 Flow Cytometry Assay Kit, Thermo Fisher) and incubated for 20min at room temperature. .. After washing, cells were resuspended in Perm/Wash buffer containing antiCD16/32 antibody, PE-conjugated NP (Bioresearch), anti-CD95-PE-Cy7 (clone, SA367H8, Biolegend), and anti-BrdU-APC (APC BrdU Flow Kit, BD Biosciences, Cat. 557892) and incubated for 30min at room temperature.

    Article Title: Maf expression in B cells restricts reactive plasmablast and germinal center B cell expansion
    Article Snippet: After washing with 2 mL BD Perm/Wash (BD Biosciences, Cat. 554723), cells were incubated in 150 μL permeabilization buffer plus (BD Biosciences, Cat. 561651) on ice for 10 min followed by wash, and 5 min fixation with 150 μL Cytofix/Cytoperm followed by another wash. For DNA digestion, cells were incubated at 37 °C for 1 h in a solution of DNase I at 300 μg/mL in PBS. .. After washing with Perm/Wash, cells were resuspended in EdU Click-iT reaction mixture (Click-iT Plus EdU Alexa 350 Flow Cytometry Assay Kit, Thermo Fisher) and incubated for 20 min at room temperature. .. After washing, cells were resuspended in Perm/Wash buffer containing anti-CD16/32 antibody, PE-conjugated NP (Bioresearch), anti-CD95-PE-Cy7 (clone, SA367H8, Biolegend), and anti-BrdU-APC (APC BrdU Flow Kit, BD Biosciences, Cat. 557892) and incubated for 30 min at room temperature.



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